Exceptional stability at ambient temperature - Stability TAG Technology
All enzymes produced at Solis BioDyne are exceptionally stable at ambient temperature due to a specific genetic modification - Stability TAG. Stability TAG increases polypeptide self life and its tolerance for temperature at a wider scale without compromising the properties of the polypeptide itself. After being stored at ambient temperature for one month (tested up to +35°C), the enzyme remained fully active.
Stability TAG Technology is now applied in all of Solis BioDynes products providing the client with enzymes, end-point Master Mixes and qPCR Mixes with exceptional stability at ambient temperature.
on a variety of proteins
stabilizing effect of Stability TAG has been tested on a variety of proteins
including Taq DNA Polymerase, hEPO, PCNA, pyrophosphatase and hLIF.
Stability TAG does not effect the properties of the protein
number of tests with different Stability TAG attached proteins revealed no affect
on protein properties. For example, pluripotency was maintained in ES cells
during gene targeting using Stability TAG-hLIF; amplification of DNA using Stability TAG-Taq DNA Polymerase (FIREPol® DNA Polymerase) was performed with the same efficiency and accuracy as
with regular Taq.
at +35ºC does not effect enzyme performance
Our products have been tested for stability at +35°C. Our enzymes FIREPol® and HOT
FIREPol® DNA Polymerase exhibited no alteration in performance even
after four weeks at +35°C (Figure A). Also no change in performance was detected between qPCR Mix stored at -20°C and the mix stored at +35°C (Figure B).
Long term stability at -20ºC
addition to excellent stability at ambient temperature, enzymes produced at
Solis BioDyne also exhibit extraordinarily long shelf life. Even after 18 years, our first batch of FIREPol® DNA Polymerase gives satisfactory results in
PCR (Figure C).
Please contact us for additional questions!
Download Stability TAG Technology flyer from here!