- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination
- Bio-Rad: CFX96™ & CFX384™, iQ™5 & MyiQ™, Chromo4™, Opticon® 2 & MiniOpticon®
- Qiagen: Rotor-Gene® Q, Rotor-Gene® 6000
- Eppendorf: Mastercycler®: ep realplex2 & ep realplex4
- Illumina: The Eco™
- Roche: LightCycler® 480
- HOT FIREPol® DNA Polymerase
- 5x Probe qPCR buffer
- 15 mM MgCl2 1x PCR solution – 3 mM
- dNTPs, including dTTP to improve reaction sensitivity and efficiency compared to dUTP
- No ROX dye
Shipping and Storage Conditions:
Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® Probe qPCR Mix Plus (no ROX).
Mastercycler is a trademark of Eppendorf AG. Rotor-Gene is a registered trademark of Corbett Research (Qiagen). CFX96, CFX384, iQ, MyiQ, Chromo4, Opticon 2, MiniOpticon are trademarks of Bio-Rad Laboratories. LightCycler is a registered trademark of Roche Applied Sciences. The Eco is registered trademark of Illumina Inc.
Please browse through our Troubleshooting Guide for qPCR
contact technical support via skype: support.sbd. All inquiries will be responded within 24 hours at most.
References of 5x HOT FIREPol® Probe qPCR Mix
Effect of COMT Val158Met polymorphism on personality traits and educational attainment in a longitudinal population representative study
Lehto K, Akkermann K, Parik J, Veidebaum T, Harro J
European Psychiatry 2013 Oct. Vol. 28. Iss. 8. pp 492-498
Triglyceride metabolism in bone tissue is associated with osteoblast and osteoclast differentiation: a gene expression study
Dragojevic J, Zupan J, Haring G, Herman S, Komadina R, Marc J
J Bone Miner Metab. 2013. 31:512-519
Increased placental expression and maternal serum levels of apoptosis-inducing TRAIL in recurrent miscarriage
Rull K, Tomberg K, Kõks S, Männik J, Möls M, Sirotkina M, Värv S, Laan M.
Placenta. 2013 Feb. Vol 34. Iss 2. pp141-148
The relationship between osteoclastogenic and anti-osteoclastogenic pro-inflammatory cytokines differs in human osteoporotic and osteoarthritic
Zupan J, Komadina R, Marc J.
J. of Biomed. Science. 2012. 19:28
Mid-Gestational Gene Expression Profile in Placenta and Link to Pregnancy Complications
Uusküla L, Männik J, Rull K, Minajeva A, Kõks S, Vaas P, Teesalu P, Reimand J, Laan M
Plos One 2012, DOI: 10.1371/journal.pone.0049248
Proteome changes of human bronchial epithelial cells in response to pro-inflammatory mediator leukotriene E4 and pro-remodelling factor TGF-beta1.
Altraja S, Jaama J, Altraja A.
J Proteomics. 2010 Apr 18;73(6):1230-40. Epub 2010 Feb 26.
Micro RNAexpression profiles of human blood monoyte-derived dendric cells and macrophages reveal miR-511 as a putative positive regulator of TLR4.
Tserel L, Runnel T, Kisand K, Pihlap M, Bakhoff L, Kolde R, PetersonH, Vilo J, Peterson P, Rebane A.
Journal of Biological Chemistry. 2011 June. 286 (30).