5x HOT FIREPol® Multiplex qPCR Mix (ROX)

For in vitro use only

 

Description:

HOT FIREPol® Multiplex qPCR Mix (ROX) is optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays. The qPCR Mix comprises all the components necessary (except primers, probes and template) to perform qPCR: HOT FIREPol® DNA Polymerase, optimized buffer components, ultrapure dNTPs, MgCl2 and ROX passive reference dye according to system requirements.

HOT FIREPol® Multiplex qPCR Mix (ROX) is optimized for DNA hydrolysis probes based on the 5' flap endonuclease activity.

HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup. 

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Cat. No. Pack size Conc. Data sheet MSDS CoA Price Product qty
08-02-000011 ml5xPDFPDFPDF107 EUR Login to buy!
08-02-000088 ml5xPDFPDFPDF856 EUR Login to buy!
08-02-0002020 ml5xPDFPDFPDF1920 EUR Login to buy!

Applications:

- Detection and quantification of DNA and cDNA targets

- Profiling gene expression

- Microbial detection

- Viral load determination

 

Reagents Provided:

- HOT FIREPol® DNA Polymerase

- 5x Multiplex qPCR buffer

- 15 mM MgCl2, 1x PCR solution – 3 mM MgCl2

- dNTPs, including dUTP The mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Multiplex qPCR Mix and must be purchased separately.

- ROX dye If ROX, Texas Red or similar dye is used as one of the fluorophores, ROX passive reference dye might interfere with the signal. A version without passive reference or with Purple reference dye is available.

 

Shipping and Storage Conditions:

Routine storage: -20 ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.



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Sample size: 0.2 ml | 5x

Order free sample

For research use only.
No components from animal or human origin

Some applications this product is used in may require a license which is not provided by the purchase of this product. Users should obtain the license if required.