5x FIREPol® Master Mix Ready to Load

with 7.5 mM MgCl2

For in vitro use only



5x FIREPol® Master Mix is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water). Ready to Load format also includes a compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) that allow to monitor progress during electrophoresis.

We recommend using 5x FIREPol® Master Mix Ready to Load in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining. 5x FIREPol® Master Mix Ready to Load is not recommended for use in post-PCR applications where spectro-photometric measurements (absorbance or fluorescence) are necessary because yellow and blue dyes can interfere with these applications.


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Cat. No. Pack size Conc. Data sheet MSDS CoA Price Product qty
04-12-001151 ml7.5 mM MgCl2PDFPDFPDF17.5 EUR Login to buy!


- Suited for a wide range of PCR assays

- Primer extension


Reagent Composition:

- FIREPol® DNA polymerase

- 5x Reaction Buffer B 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20 

- 7.5 mM MgCl2 1x PCR solution – 1.5 mM MgCl2 

- 2 mM dNTPs of each  1x PCR solution – 200 µM dATP, 200 µM dCTP,   200 µM dGTP and 200 µM dTTP

- Blue dye Migration equivalent to 3.5-4.5 kb DNA fragment

- Yellow dye  Migration rate in excess of primers in 1% agarose gel: <35-45 bp

- Compound that increases sample density for direct loading

Advantages of Ready-to-Load Mix

- decreased set-up time

- reduced risk of contamination

- less pipetting mistakes

- consistent reaction to reaction performance

- contains all reagents required for PCR

Like all our products, the 5x FIREPol® Master Mix Ready to Load is stable at ambient temperature for up to one month. This makes handling and set-up even more convenient, since no ice has to be used.

Shipping and Storage Conditions:

Routine storage: -20şC
Shipping and temporary storage for up to 1 month at room temperature or storage for up to 6 months at 2-8şC has no detrimental effects on the quality of FIREPol® 5x Master Mix Ready to Load.

Please browse through our Troubleshooting Guide for End-Point PCR

send an e-mail directly to our technical support or 

contact technical support via skype: support.sbd. All inquiries will be responded within 24 hours at most.

References of FIREPol® Master Mix

Analysis of Clinical Isolates of Helicobacter pylori in Pakistan Reveals High Degrees of Pathogenicity and High Frequencies of Antibiotic Resistance
Rasheed F, Campbell BJ, AlfizahH, Varro A, Zahra R, Yamaoka Y,  Pritchard D
Heliobacter, 2014, Online, DOI:10.1111/hel.12142

Differential Endosomal Pathways for Radically Modified Peptide Vectors
Arukuusk P, Pärnaste L, Margus H, Eriksson NKJ, Vasconcelos L, Padari K, Pooga M, Langel L
Bioconjugate Chem., 2013, 24 (10), pp 1721–1732

CCL5/CCR1 axis regulates multipotency of human adipose tissue derived stromal cells
Kauts ML, Pihelgas S, Orro K, Neuman T, Piirsoo A
Stem Cell Research, 2013, Vol. 10, Iss. 2, Pp 166–178

Sample size: 0.10 ml | 7.5 mM MgCl2

Order free sample

For research use only.
No components from animal or human origin

Some applications this product is used in may require a license which is not provided by the purchase of this product. Users should obtain the license if required.



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