name

FIREPol® DNA Polymerase

 For in vitro use only

 

Description:

FIREPol® is a highly processive, thermostable DNA polymerase. Due to its genetic modifications FIREPol® has an enhanced stability at room temperature with no activity loss for up to 1 month. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity.

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Cat. No. Pack size Conc. Data sheet MSDS CoA Price Product qty
01-01-00500500 U5 U/μlPDFPDFPDF14.5 EUR Login to buy!
01-01-010001000 U5 U/μlPDFPDFPDF29 EUR Login to buy!
01-01-020002000 U5 U/μlPDFPDFPDF58 EUR Login to buy!

Applications:

- Suited for a wide range of PCR assays

- Primer extension

- TA cloning


Concentration:

5 U/µl


Storage and Dilution Buffer:

50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.

 

Reagents Provided:

- FIREPol® DNA Polymerase

- 10x Reaction buffer B (Mg2+ free) 

0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20

- 10x Reaction buffer BD (Mg2+ and detergent free)

0.8 M Tris-HCl, 0.2 M (NH4)2SO4

-  25 mM MgCl2

- 10x Solution S Additive that facilitates amplification of difficult templates (e.g. GC-rich DNA templates). This solution should be used at a defined working concentration (1x, 2x or 3x solution).
Solution S is NOT a reaction buffer and should be used ONLY IF non-specific amplifications occur.


Shipping and Storage Conditions:

Routine storage: -20 ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of FIREPol® DNA Polymerase.



Please browse through our Troubleshooting Guide for End-Point PCR

send an e-mail directly to our technical support or 

contact technical support via skype: support.sbd. All inquiries will be responded within 24 hours at most.

References of FIREPol® DNA Polymerase


Development of an event-specific assay for the qualitative and quantitative detection of the genetically modified flax CDC Triffid (FP967)
Vanella R, Weston A, Brodmann P, Kübler E
Food Control, 2014. Vol. 41. Pp 128-133

5-HT2A and mGlu2 receptor binding levels are related to differences in impulsive behavior in the Roman Low- (RLA) and High- (RHA) avoidance rat strains
Kleina AB et al.
Neuroscience, 2014, Vol. 263, Pp 36–45

Molecular genetic analysis of Dichelobacter nodosus proteases AprV2/B2, AprV5/B5 and BprV/B in clinical material from European sheep flocks
Stäublea A, Steiner A, Normand L, Kuhnert P, Frey J
Veterinary Microbiology, 2014. Vol. 168, Iss. 1, Pp. 177–184

SDA, a DNA Aptamer Inhibiting E- and P-Selectin Mediated Adhesion of Cancer and Leukemia Cells, the First and Pivotal Step in Transendothelial Migration during Metastasis Formation
Faryammanesh R, Lange T, Magbanua E, Haas S, Meyer C, Wicklein D, Schumacher U, Hahn U 
Plos One, 2014, DOI: 10.1371/journal.pone.0093173

Improved PCR/nested PCR approaches with increased sensitivity and specificity for the detection of pathogens in hard ticks
Kim EJ, Bauer C, Grevelding CG, Quack T
Ticks and Tick-borne Diseases, 2013, Vol. 4, Iss. 5, Pp. 409–416

Development and characterization of 21 microsatellite markers for Pancratium maritimum L. (Amyllidaceae)
Di Maigo A, De Castro O
Conservation Genet. Resour , 2013, 5:911-914

PCR Typing of Mycobacterium ulcerans Lineages Based on Large Sequence Polymorphisms
Kaser M, Hauser J, Pluschke G
J Medical Microbiol Diagnosis. 2012 Jan

Genotypic and phenotypic diversity in Finnish cultivated sour cherry (Prunus cerasus L.)
Antonius K, Aaltonen M, Uosukainen M, Hurme T.
Genetic Resources and Crop Evolution. 2012, Vol. 59, No. 3, Pp.  375-388

Passive Immunoprotection of Plasmodium falciparum-Infected Mice Designates the CyRPA as Candidate Malaria Vaccine Antigen
Dreyer AM, Matile H, Papastogiannidis P, Kamber J, Favuzza P, Voss TS,  Wittlin S, Pluschke G
The Journal of Immunology. 2012 June, Vol. 188, No. 12, Pp. 6225-6237

Genetically modified Bt maize lines containing cry3Bb1, cry1A105 or cry1Ab2 do not affect the structure and functioning of root-associated endophyte communities
Prischl M, Hackl E, Pastar M, Pfeiffer S, Sessitsch A
Applied Soil Ecology. 2012, Vol. 54, Pp. 39–48

Rapid and dissimilar response of ammonia oxidizing archaea and bacteria to nitrogen and water amendment in two temperate forest soils
Szukicsa U, Hackla E, Zechmeister-Boltensternb S, Sessitscha A
Microbiological Research, January 2012, Vol. 167, Iss.2, Pp. 103–109

The effector SPRYSEC-19 of Globodera rostochiensis suppresses CC-NB-LRR-mediated disease resistance in plants
Postma W. et al.  
Plant Physiology,  2012 Aug. Pp. 112.200188

Sample size: 100.00 U | 5 U/μl

Order free sample

For research use only.
No components from animal or human origin

Some applications this product is used in may require a license which is not provided by the purchase of this product. Users should obtain the license if required.

Contact:

E-mail: solis@sbd.ee

Tel: +372 740 9960

Skype: solis.biodyne Skype Meâ„¢!

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