Applications:
- Suited for a wide range of PCR assays
- Primer extension
- TA cloning
Concentration:
5 U/µl
Storage and Dilution Buffer:
50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.
Reagents Provided:
- FIREPol® DNA Polymerase
- 10x Reaction buffer B (Mg2+ free)
0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20
- 10x Reaction buffer BD (Mg2+ and detergent free)
0.8 M Tris-HCl, 0.2 M (NH4)2SO4
- 25 mM MgCl2
- 10x Solution S
Additive that facilitates amplification of
difficult templates (e.g. GC-rich DNA templates). This solution should
be used at a defined working concentration (1x, 2x or 3x solution).
Solution S is NOT a reaction buffer and should be used ONLY IF non-specific amplifications occur.
Shipping and Storage Conditions:
Routine storage: -20 ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of FIREPol® DNA Polymerase.
References of FIREPol® DNA Polymerase
Development of an event-specific assay for the qualitative and quantitative detection of the genetically modified flax CDC Triffid (FP967)
Vanella R, Weston A, Brodmann P, Kübler E
Food Control, 2014. Vol. 41. Pp 128-133
5-HT2A and mGlu2 receptor binding levels are related to differences in impulsive behavior in the Roman Low- (RLA) and High- (RHA) avoidance rat strains
Kleina AB et al.
Neuroscience, 2014, Vol. 263, Pp 36–45
Molecular genetic analysis of Dichelobacter nodosus proteases AprV2/B2, AprV5/B5 and BprV/B in clinical material from European sheep flocks
Stäublea A, Steiner A, Normand L, Kuhnert P, Frey J
Veterinary Microbiology, 2014. Vol. 168, Iss. 1, Pp. 177–184
SDA, a DNA Aptamer Inhibiting E- and P-Selectin Mediated Adhesion of Cancer and Leukemia Cells, the First and Pivotal Step in Transendothelial Migration during Metastasis Formation
Faryammanesh R, Lange T, Magbanua E, Haas S, Meyer C, Wicklein D, Schumacher U, Hahn U
Plos One, 2014, DOI: 10.1371/journal.pone.0093173
Improved PCR/nested PCR approaches with increased sensitivity and specificity for the detection of pathogens in hard ticks
Kim EJ, Bauer C, Grevelding CG, Quack T
Ticks and Tick-borne Diseases, 2013, Vol. 4, Iss. 5, Pp. 409–416
Development and characterization of 21 microsatellite markers for Pancratium maritimum L. (Amyllidaceae)
Di Maigo A, De Castro O
Conservation Genet. Resour , 2013, 5:911-914
PCR Typing of Mycobacterium ulcerans Lineages Based on Large Sequence Polymorphisms
Kaser M, Hauser J, Pluschke G
J Medical Microbiol Diagnosis. 2012 Jan
Genotypic and phenotypic diversity in Finnish cultivated sour cherry (Prunus cerasus L.)
Antonius K, Aaltonen M, Uosukainen M, Hurme T.
Genetic Resources and Crop Evolution. 2012, Vol. 59, No. 3, Pp. 375-388
Passive Immunoprotection of Plasmodium falciparum-Infected Mice Designates the CyRPA as Candidate Malaria Vaccine Antigen
Dreyer AM, Matile H, Papastogiannidis P, Kamber J, Favuzza P, Voss TS, Wittlin S, Pluschke G
The Journal of Immunology. 2012 June, Vol. 188, No. 12, Pp. 6225-6237
Genetically modified Bt maize lines containing cry3Bb1, cry1A105 or cry1Ab2 do not affect the structure and functioning of root-associated endophyte communities
Prischl M, Hackl E, Pastar M, Pfeiffer S, Sessitsch A
Applied Soil Ecology. 2012, Vol. 54, Pp. 39–48
Rapid and dissimilar response of ammonia oxidizing archaea and bacteria to nitrogen and water amendment in two temperate forest soils
Szukicsa U, Hackla E, Zechmeister-Boltensternb S, Sessitscha A
Microbiological Research, January 2012, Vol. 167, Iss.2, Pp. 103–109
The effector SPRYSEC-19 of Globodera rostochiensis suppresses CC-NB-LRR-mediated disease resistance in plants
Postma W. et al.
Plant Physiology, 2012 Aug. Pp. 112.200188