SOLIScript 1-step Multiplex Probe Kit (ROX)

For in vitro use only

 

Description:

SOLIScript 1-step Multiplex Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays. It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube. 

Reagent composition is optimized for excellent performance in highly multiplexed reactions while making sure you don't lose in sensitivity compared to singleplex or duplex assays.

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Cat. No. Pack size Conc. Data sheet MSDS CoA Price Product qty
08-59-00250250 rxnPDFPDF425 EUR Login to buy!

Applications:

- Detection and quantification of RNA targets with qPCR

- DNA/LNA hydrolysis probe based assays

- Suitable for singleplex and multiplex reactions

 

Reagents Provided:

- 5x One-step Multiplex Probe Mix (ROX)

  HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP), 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2), ROX dye

  If ROX dye is used as one of the fluorophpres, internal passive reference dye might interfere with the signal. A version without passive reference or with Purple reference dye is available.

- 40x One-step SOLIScript Mix

  SOLIScript Reverse Transcriptase, RiboGrip RNase Inhibitor

- Water, nuclease free

 

Shipping and Storage Conditions:

Routine storage: -20 ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.



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or contact technical support via skype: support.sbd

Sample size: 50 rxn

Order free sample

For research use only.
No components from animal or human origin

Some applications this product is used in may require a license which is not provided by the purchase of this product. Users should obtain the license if required.