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M-MLV Reverse Transcriptase RNase H-

 

For in vitro use only

Description:

The M-MLV Reverse Transcriptase RNase H- is a genetically modified M-MLV Reverse Transcriptase which exhibits RNA or DNA dependent DNA polymerase, but lacks ribonuclease H activity. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA or DNA templates. Removal of RNase H activity results in an increase of full-length cDNA products

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Cat. No. Pack size Conc. Data sheet MSDS CoA Price Product qty
06-21-01000010000 U200 U/μlPDFPDFPDF60 EUR Login to buy!
06-21-05000050000 U200 U/μlPDFPDFPDF300 EUR Login to buy!
06-21-200000200000 U200 U/μlPDFPDFPDF1000 EUR Login to buy!

Applications:

- RNA analysis by primer extension

- DNA labeling

- cDNA synthesis


Concentration:

  200U/l

 

Reagents Provided:

- M-MLV Reverse Transcriptase RNase-

- 5x Reaction Buffer 1 (With MgCl2 and DTT)
  0,25 M Tris-HCl, 0.5 M KCl, 30mM MgCl2, 25 mM DTT

- 5x Reaction Buffer 2 (MgCl2 and DTT free)
 
0,25 M Tris-HCl, 0.5 M KCl

- 5x Reaction Buffer 2 (MgCl2 and DTT free)
 
0,25 M Tris-HCl, 0.5 M KCl

- 25 mM MgCl2

- 20 mM MnCl

- 100 mM DDT

 

Storage and Dilution Buffer:

50% glycerol (v/v), 50 mM Tris-HCl pH 8.0 at 25C, 100 mM NaCl, 5mM DTT, 1 mM EDTA, 0.1 % NP-40


Shipping and Storage Conditions:

Routine storage: -20C
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of M-MLV Reverse Transcriptase  RNase H-.



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or contact technical support via skype: support.sbd

Sample size: 2000.00 U | 200 U/μl

Order free sample

For in vitro use only

Some applications this product is used in may require a license which is not provided by the purchase of this product. Users should obtain the license if required.

Contact:

E-mail: solis@sbd.ee

Tel: +372 740 9960

Skype: solis.biodyne Skype Me™!

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