5x HOT FIREPol® MultiPlex Mix Ready To Load

with 10 mM MgCl2

For in vitro use only 


5x HOT FIREPol® MultiPlex Mix RTL is a premixed ready-to-use solution containing all reagents required for hot-start multiplex PCR (except template, primers and water) and an additional compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) that allow to monitor progress during electrophoresis.
Generated PCR products are compatible with TA cloning procedures



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Cat. No. Pack size Conc. Data sheet MSDS CoA Price Product qty
04-36-001201 ml5xPDFPDFPDF33 EUR Login to buy!
04-36-0202020 ml5xPDFPDFPDF580 EUR Login to buy!


- Hot Start PCR 

- Multiplex PCR


Mix Composition:

HOT FIREPol® DNA polymerase

- 5x MultiPlex Buffer

- 10 mM MgCl2 1x PCR solution – 2 mM MgCl2

- dNTPs

- BSA 

- Blue dye  Migration equivalent to 3.5-4.5 kb DNA fragment

- Yellow dye Migration equivalent to 35-45 bp DNA fragment

- Compound that increases sample density for direct loading

Advantages of Master Mix:

- decreased set-up time 

- reduced risk of contamination 

- less pipetting mistakes 

- consistent reaction to reaction performance 

- contains all reagents required for multiplex PCR 


Like all our products, the 5x HOT FIREPol® MultiPlex Mix is stable at ambient temperature for up to one month. This makes handling and set-up even more convenient since no ice has to be used.


Product Performance:


Shipping and Storage Conditions:

Routine storage: -20ºC 
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of 5x HOT FIREPol® MultiPlex Mix.


Please browse through our Troubleshooting Guide for End-Point PCR

send an e-mail directly to our technical support or 
contact technical support via skype: support.sbd. All inquiries will be responded within 24 hours at most.

Sample size: 0.1 ml | 5x

Order free sample

For research use only.
No components from animal or human origin

Some applications this product is used in may require a license which is not provided by the purchase of this product. Users should obtain the license if required.