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5x HOT FIREPol® GC Master Mix

For in vitro use only 


Description:

5x HOT FIREPol® GC Master Mix is designed to provide highly specific high-yield amplification of GC-rich templates. Master Mix is a ready-to-use solution containing all reagents required for PCR (except template, primers and water). 100% DMSO and 25 mM MgCl2 are included in a package in separated vials.

Generated PCR products are compatible with TA cloning procedures

 

 

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Cat. No. Pack size Conc. Data sheet MSDS CoA Price Product qty
04-33-001151 ml5xPDFPDFPDF31 EUR Login to buy!
04-33-0201520 ml5xPDFPDFPDF560 EUR Login to buy!

Applications:

- Hot Start GC-rich PCR 

- Fragment analysis

- TA cloning

 

Mix Composition:

HOT FIREPol® DNA polymerase

- HOT FIREPol® GC Buffer

- 7.5 mM MgCl2 1x PCR solution – 1.5 mM MgCl2

- 2 mM dNTPs

- BSA 

 

In separate vial:

- 100% DMSO

- 25 mM MgCl2

 

Advantages of Master Mix:

- decreased set-up time 

- reduced risk of contamination 

- less pipetting mistakes 

- consistent reaction to reaction performance 

- contains all reagents required for PCR 

 

Like all our products, the 5x HOT FIREPol® GC Master Mix is stable at ambient temperature for one month. This makes handling and set-up even more convenient, since no ice has to be used.

 

Product Performance:

The unique composition of the mix supports robust amplification of broad range of GC-rich targets. A total 14 GC-rich genes were amplified from human genomic DNA, using recommended reaction set-up and cycling protocol for 5x HOT FIREPol® GC Master Mix.
 

 

 

 

 

 

 

 

 

 

 

 

Mix performs well with GC-rich  amplicons up to 3000 bp.


 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Good results are obtained also with Fragment Analyzis (DAT/SLC6A3).  
 

 

Shipping and Storage Conditions:

Routine storage: -20ºC 
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of 5x HOT FIREPol® GC Master Mix.

 

Please browse through our Troubleshooting Guide for End-Point PCR

 
send an e-mail directly to our technical support or 
 
contact technical support via skype: support.sbd. All inquiries will be responded within 24 hours at most.
 

Sample size: 0.10 ml | 5x

Order free sample

For in vitro use only

Some applications this product is used in may require a license which is not provided by the purchase of this product. Users should obtain the license if required.

Contact:

E-mail: solis@sbd.ee

Tel: +372 740 9960

Skype: solis.biodyne Skype Meā„¢!

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