Hot Start PCR



Hot Start PCR prevents misextension from non-specifically annealed primers and primer-dimers.

HOT FIREPol®  DNA Polymerase is a chemically modified FIREPol®  DNA Polymerase. At ambient temperature it is inactive having no polymerization activity. HOT FIREPol® DNA Polymerase is activated by a 12-15 min incubation step at 95ş C

Hot-start master mixes are also available in ready-to-load format with variety of MgCl2 concentrations.

5x HOT FIREPol® Blend Master Mix is a highly user-friendly and versatile tool for your Hot Start PCR. The mix is based on a blend of our HOT FIREPol® polymerase and a proofreading polymerase, providing a low error rate and high sensitivity. In addition, the mix also allows amplification of longer fragments (up to 5 kb). Here some great advantages of this ready-to-load mix:

  • decreased set-up time
  • reduced risk of contamination
  • less pipetting mistakes
  • consistent reaction to reaction performance
  • contains all reagents required for PCR

Like all our products, the 5x HOT FIREPol® Blend Master Mix is stable at ambient temperature for up to one month. This makes handling and set-up even more convenient, since no ice has to be used. 

 

 


Product Data sheet MSDS CoA
HOT FIREPol® DNA PolymerasePDFPDFPDF
5x HOT FIREPol® GC Master MixPDFPDFPDF
5x HOT FIREPol® Blend Master Mix with 7.5 mM MgCl2PDFPDFPDF
5x HOT FIREPol® Blend Master Mix with 10 mM MgCl2PDFPDFPDF
5x HOT FIREPol® Blend Master Mix with 12.5 mM MgCl2PDFPDFPDF
5x HOT FIREPol® Blend Master Mix with 15 mM MgCl2PDFPDFPDF
5x HOT FIREPol® Blend Master Mix Ready to Load with 7.5 mM MgCl2PDFPDFPDF
5x HOT FIREPol® Blend Master Mix Ready to Load with 10 mM MgCl2PDFPDFPDF
5x HOT FIREPol® Blend Master Mix Ready to Load with 12.5 mM MgCl2PDFPDFPDF
5x HOT FIREPol® Blend Master Mix Ready to Load with 15 mM MgCl2PDFPDFPDF
5x HOT FIREPol® MultiPlex Mix Ready To Load with 10 mM MgCl2PDFPDFPDF
5x HOT FIREPol® MultiPlex Mix with 10 mM MgCl2PDFPDFPDF

Contact:

E-mail: solis@sbd.ee

Tel: +372 740 9960

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